Camelina Breeding

Summary: Camelina sativa, a largely relict crop, has recently returned to interest due to its potential as an industrial oilseed. Molecular markers are key tools that will allow C. sativa to benefit from modern breeding approaches. Two complementary methodologies, capture of 3′ cDNA tags and genomic reduced-representation libraries, both of which exploited second generation sequencing platforms, were used to develop ...

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Summary: Camelina is a low-input oilseed crop of recent interest for sustainable biofuel production. As a relatively new crop in modern agriculture, considerable agronomic and regulatory problems need to be overcome. A common and troublesome problem is sensitivity to residues of acetolactate synthase (ALS) inhibitor herbicides in soils. An allele associated with the highest levels of resistance was created by ...

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    Summary: Camelina [Camelina sativa (L.) Crtz., Brassicaceae], known as false flax or gold-of-pleasure is a spring-planted crop species. Although camelina has been cultivated in Europe since the Bronze Age, it is an underexploited oilseed crop at present. In the present investigation, results from an agronomic evaluation of new lines of camelina are reported. The genotypes tested were derived ...

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Summary: A set of 130 camelina accessions from a world collection was evaluated for oil content, protein content and 1000-seed weight in field experiments grown in three macro-environments in Austria. In addition, a representative set of 41 accessions was subjected to random amplified polymorphic DNA analysis. Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2005.01134.x/abstract

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Summary: To identify candidate genes for meal and oil quality improvement, a transcriptome reference was built from 2047 Sanger ESTs and more than 2 million 454-derived sequence reads, representing genes expressed in developing camelina seeds. These transcriptomic data will be useful for breeding and engineering of additional camelina seed traits and for translating findings from the model Arabidopsis to an ...

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Summary: A genomic DNA library enriched with GA/TC repeats from Camelina sativa variety Calena has been analysed. Link: http://link.springer.com/article/10.1007%2Fs10722-012-9913-8#page-1

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Summary: This is the first report of a transcriptome database for Camelina sativa. We showed that C. savita is closely related to Arabidopsis spp. and more distantly related to Brassica spp. Although the majority of annotated genes had high sequence identity to those of A. thaliana, a substantial proportion of disease-resistance genes  were instead more closely similar to the genes ...

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Summary: We generated the first chromosome-scale high-quality reference genome sequence for C. sativa and annotated 89,418 protein-coding genes, representing a whole-genome triplication event relative to the crucifer model Arabidopsis thaliana. C. sativa represents the first crop species to be sequenced from lineage I of the Brassicaceae. The well-preserved hexaploid genome structure of C. sativa surprisingly mirrors those of economically important ...

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Summary: There is compelling evidence for triplication of the C. sativa genome, including a larger chromosome number and three-fold larger measured genome size than other Camelina relatives, three isolated copies of FAD2, FAE1, and the KCS17-FAE1 intergenic region, and three expressed haplotypes observed for six predicted single-copy genes. Based on these results, we propose that C. sativa be considered an ...

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Summary: We investigated the role of geographical origin in genetic variation and fatty acid content, expecting to find significant variability among 53 accessions and a link between ecogeography and both origin and key oil traits. Although sampling was relatively biased towards the Russian–Ukrainian area, this region was identified as a genetic diversity hotspot and possible centre of origin for camelina. ...

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Summary: In this study, a genetic map for C. sativa was constructed, using amplified fragment length polymorphism markers, in a population of recombinant inbred lines. The map was used to localize quantitative trait loci for agronomic characteristics, such as seed yield, oil content, 1000-seed weight, and plant height. The results represent a starting point for future marker-assisted breeding. Link: http://www.ncbi.nlm.nih.gov/pubmed/17426770

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Summary: We report a new set of nine primer pairs specifically developed for amplification of Brassica plastid SSR markers. The analysis was generally able to separate plastid types into taxon-specific groups. We interpret our results with respect to taxon differentiation, hybridisation and introgression patterns relating to the ‘Triangle of U’.  Link: http://link.springer.com/article/10.1007%2Fs00122-006-0377-0#page-1

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Summary: Cruciferous seed oils from the crops rapeseed, mustard, Camelina, oilseed radish and Crambe, enter edible or industrial markets, or both. In the next 10 to 15 years, application of newer plant-breeding techniques will result in varieties even higher in yield and seed with improved oil and meal quality. Link: http://link.springer.com/article/10.1007%2FBF02638528#page-1

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In this study, seeds of the German C. sativa cultivar Lindo were mutagenically treated with ethyl methanesulfonate to modify the fatty acid composition in the seed oil and to select mutants with either reduced or increased linolenic acid content, respectively. Link: http://www.sciencedirect.com/science/article/pii/S0926669097000605

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